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Jun 01, 2021 · The next day, 175 µl RIPA/140 mM NaCl buffer (0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100,140 mM NaCl, 1 mM EDTA, 20 mM Tris-HCl [pH 8.1]) was added to each tube and beads were transferred to a 96-well plate on a magnet, washed twice with cold RIPA/500 ml NaCl buffer (0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 140 mM NaCl, 1 ...

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BIOGRAPHICAL J. MORGAN DONELSON Grand Master 1969-1970. J. Morgan Donelson was elected Grand Master of the Grand Lodge of Missouri September 29, 1969, and was installed as its l24th Grand Master ...

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The Subaru EJ20Y and EJ20X were turbocharged, 2.0-litre horizontally-opposed (or 'boxer') four-cylinder petrol engines. For Australia, the EJ20X engine was introduced in the 2003 Subaru BL Liberty GT and mated to a five-speed automatic transmission; the EJ20Y was subsequently introduced in 2004 and had a five-speed manual transmission. The EJ20Y and EJ20X engines …

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LT140 IDLER H14 RIPA D=320/70 cs cone crusher piston wearing plate dimensions

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